ABM Mushroom Research
RESEARCH JOURNAL REFERENCES
& LISTS for AGARICUS BLAZEI MURILL
Revised 19 June 2001 - this list will be updated periodically
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Mizuno T, Inagaki R, Kanao T,
Hagiwara T, Nakamura T, Ito H, Shimura K, Sumiya T, Asakura A
Antitumor Activity and Some Properties of Water-insoluble
Hetero-glycans from "Himematsutake," the Fruiting Body of Agaricus
blazei Murill.
Agricultural and Biological Chemistry; 54(11):2889-2896 1990
ABM MUSHROOM RESEARCH - JOURNAL REFERENCE ITEM 8:
AUTHOR:
Mizuno T, Inagaki R, Kanao T, Hagiwara T, Nakamura T, Ito H, Shimura K, Sumiya
T, Asakura A
TITLE:
Antitumor Activity and Some Properties of Water-insoluble Hetero-glycans from
"Himematsutake," the Fruiting Body of Agaricus blazei Murill.
JOURNAL:
Agricultural and Biological Chemistry; 54(11):2889-2896 1990
ADDRESS:
Faculty of Agriculture, Shizuoku Univ., Ohya, Shizuoku 422, Japan
ABSTRACT:
After extraction of a hot-water-soluble polysaccharide (FI) from the fruiting
bodies of Himematsutake (Agaricus blazei Murill), water-insoluble
polysaccharides were obtained by successive extraction with 1% ammonium oxalate
solution (FII), 5% sodium hydroxide solution (FIII and FIV), 20% sodium
hydroxide solution (FV), and 5% lithium chloride-dimethylacetamide solution (FVI)
in that order. These water-insoluble fractions were futher fractionated by
ethanol precipitation, gel-filtration, etc. Polysaccharides,
polysaccharide-protein complexes, and chitin substances thus obtained were
assayed for their anti-tumor activities using the Sarcoma 180/mice i.p. o.p.
method
The heteroglycan-protein complexes, FII-a,-b,-c, obtained from FII had weak
antitumor activities.
A remarkable antitumor activity was found in a glycoprotein, FIII-2-b,
fractionated and purified from FIII. The polysaccharide portion of this
polysaccharide-protein complex (polysaccharide, 50.2% and protein, 43.3% each on
a weight basis) consisted of (1--6)-beta-D-glucan, and its protein portion was
rich in Asx, Glx, Ala, Leu, and Pro.
A high antitumor activity was found in a xyloglucan-protein complex, FIV-2-b,
fractionated and purified from FIV.
Antitumor activity was found also in a glucoxylan, FV-2-a, obtained from FV.
No significant antitumor activity was found in a chitin substance, FVI.
Mizuno T, Hagiwara T, Nakamura T, Ito H, Shimura K, Sumiya T, Asakura A
Antitumor Activity and Some Properties of Water-soluble Polysaccharides from
"Himematsutake," the Fruiting Body of Agaricus blazei Murill.
Agricultural and Biological Chemistry; 54(11):2897-2906 1990
ABM MUSHROOM RESEARCH - JOURNAL REFERENCE ITEM 7:
AUTHOR:
Mizuno T, Hagiwara T, Nakamura T, Ito H, Shimura K, Sumiya T, Asakura A
TITLE:
Antitumor Activity and Some Properties of Water-soluble Polysaccharides from
"Himematsutake," the Fruiting Body of Agaricus blazei Murill.
JOURNAL:
Agricultural and Biological Chemistry; 54(11):2897-2906 1990
ADDRESS:
Faculty of Agriculture, Shizuoku Univ., Ohya, Shizuoku 422, Japan
ABSTRACT:
Polysaccharides extracted from Himematsutake, the fruiting body of Agaricus
blazei Murill with hot water were fractionated and purified by ethanol
precipitation, ion-exchange chromatography, gel-filtration, affinity
chromatography, etc.
A total of 17 polysaccharide samples thus obtained were given an antitumor
activity test (Sarcoma 180/mice i.p. p.o. method) and traces of their activities
through the fractionation and purification processes were found.
FI0-a-beta, FA-1-a-alpha, FA-1-a-beta, and FA-2-b-beta, were obtained as water
soluble polysaccharides fractions having great antitumor activities.
Analyses of physico-chemical properties and IR- and NMR-spectra of the these
active fractions showed that their main components were: FI0-a-beta, (1--6)-;
(1--3)-beta-D-glucan; FA-1-a-alpha, acidic (1--6)-; (1--4)-alpha-D-glucan;
FA-1-a-beta, acidic (1--6)-; (1--3)-alpha-D-glucan; and FA-2-b-beta, acidic
RNA-protein complex.
Fujimiya Y, Suzuki Y, Oshiman K, Kobori H, Moriguchi K, Nakashima H, Matumoto Y,
Takahara S, Ebina T, Katakura R
Selective Tumoricidal Effect of Soluble Proteoglucan Extracted from the
Basidiomycete, Agaricus blazei Murill, Mediated via Natural Killer Cell
Activation and Apoptosis.
Cancer Immunology Immunother; 46(3):147-59 1998
ABM MUSHROOM RESEARCH - JOURNAL REFERENCE ITEM 1:
AUTHOR:
Fujimiya Y, Suzuki Y, Oshiman K, Kobori H, Moriguchi K, Nakashima H, Matumoto Y,
Takahara S, Ebina T, Katakura R
TITLE:
Selective Tumoricidal Effect of Soluble Proteoglucan Extracted from the
Basidiomycete, Agaricus blazei Murill, Mediated Via Natural Killer Cell
Activation and Apoptosis.
JOURNAL:
Cancer Immunol Immunother; 46(3):147-59 1998
ADDRESS:
Division of Immunology, Miyagi Cancer Center Research Institute, Natori, Japan.
ABSTRACT:
We have isolated a novel type of natural tumoricidal product from the
basidiomycete strain, Agaricus blazei Murill. Using the double-grafted tumor
system in Balb/c mice, treatment of the primary tumor with an acid-treated
fraction (ATF) obtained from the fruit bodies resulted in infiltration of the
distant tumor by natural killer (NK) cells with marked tumoricidal activity. As
shown by electrophoresis and DNA fragmentation assay, the ATF also directly
inhibited tumor cell growth in vitro by inducing apoptotic processing; this
apoptotic effect was also demonstrated by increased expression of the Apo2.7
antigen on the mitochondrial membranes of tumor cells, as shown by flow-cytometric
analysis. The ATF had no effect on normal mouse splenic or interleukin-2-treated
splenic mononuclear cells, indicating that it is selectively cytotoxic for the
tumor cells. Cell-cycle analysis demonstrated that ATF induced the loss of S
phase in MethA tumor cells, but did not affect normal splenic mononuclear cells,
which were mainly in the G0G1 phase. Various chromatofocussing purification
steps and NMR analysis showed the tumoricidal activity to be chiefly present in
fractions containing (1-->4)-alpha-D-glucan and (1-->6)-beta-D-glucan,
present in a ratio of approximately 1:2 in the ATF (molecular mass 170 kDa),
while the final purified fraction, HM3-G (molecular mass 380 kDa), with the
highest tumoricidal activity, consisted of more than 90% glucose, the main
component being (1-->4)-alpha-D-glucan with (1-->6)-beta branching, in the
ratio of approximately 4:1.
Recent
Clinical
Trial in China at the largest teaching
Hospital - Med School in the world with approximately 37,000 students!
Research
in Japan
RESEARCH JOURNAL REFERENCE
LIST for AGARICUS BLAZEI MURILL
1 Fujimiya Y, Suzuki Y, Oshiman K, Kobori H, Moriguchi K,
Nakashima H, Matumoto Y, Takahara S, Ebina T, Katakura R Selective Tumoricidal
Effect of Soluble Proteoglucan Extracted from the Basidiomycete, Agaricus
blazei Murill, Mediated via Natural Killer Cell Activation and Apoptosis.
Cancer Immunology Immunother; 46(3):147-59 1998 Link
to Abstract 2 Ito H, Shimura K, Itoh H, Kawade M Antitumor Effects of a New
Polysaccharide-protein Complex (ATOM) Prepared from Agaricus blazei (Iwade
Strain 101) "Himematsutake" and Its Mechanisms in Tumor-bearing Mice.
Anticancer Research; 17(1A):277-84 1997 Link
to Abstract 3 Itoh H, Ito H, Amano H, Noda H Inhibitory Action of a
(1-->6)-Beta-D-glucan-protein Complex (F III-2-b) Isolated from Agaricus
blazei Murill ("Himematsutake") on Meth A Fibrosarcoma-bearing
Mice and Its Antitumor Mechanism. Japan Journal of Pharmacology; 66(2):265-71
1994 Link to Abstract 4
Osaki Y, Kato T, Yamamoto K, Okubo J, Miyazaki T Antimutagenic and Bactericidal
Substances in the Fruit Body of a Basidiomycete Agaricus blazei, Jun-17.
Yakugaku Zasshi; 114(5):342-50 1994 Link
to Abstract 5 Kawagishi H, Inagaki R, Kanao T, Mizuno T, Shimura K, Ito H,
Hagiwara T, Nakamura T Fractionation and Antitumor Activity of the
Water-insoluble Residue of Agaricus blazei Fruiting Bodies. Carbohydrate
Research; 186(2):267-73 1989 Link
to Abstract 6 Shimura K, Ito H, Hibasami H Screening of Host-mediated
Antitumor Polysaccharides by Crossed Immunoelectrophoresis Using Fresh Human
Serum. Japan Journal of Pharmacology; 33(2):403-8 1983 Link
to Abstract 7 Mizuno T, Hagiwara T, Nakamura T, Ito H, Shimura K, Sumiya T,
Asakura A Antitumor Activity and Some Properties of Water-soluble
Polysaccharides from "Himematsutake," the Fruiting Body of Agaricus
blazei Murill. Agricultural and Biological Chemistry; 54(11):2897-2906 1990 Link
to Abstract 8 Mizuno T, Inagaki R, Kanao T, Hagiwara T, Nakamura T, Ito H,
Shimura K, Sumiya T, Asakura A Antitumor Activity and Some Properties of
Water-insoluble Hetero-glycans from "Himematsutake," the Fruiting Body
of Agaricus blazei Murill. Agricultural and Biological Chemistry;
54(11):2889-2896 1990 Link
to Abstract 9 Kawagishi H, Kanao T, Inagaki R, Mizuno T, Shimura K, Ito H,
Hagiwara T, Nakamura T Formolysis of a Potent Antitumor
(1-6)-beta-D-Glucan-Protein Complex from Agaricus blazei Fruiting Bodies
and Antitumor Activity of the Resulting Products. Carbohydrate Polymers;
12(4):393-404 1990 Link to
Abstract 10 Kawagishi H, Inagaki R, Kanao T, Mizuno T, Shimura K, Ito H
Hagiwara T, Nakamura T Fractionation and Antitumor Activity of the Water-soluble
Residue of Agaricus blazei Fruiting Bodies. Carbohydrate Research;
186:267-273 1989 11 Kawagishi H, Katsumi R, Sazawa T, Mizuno T, Hagiwara T,
Nakamura T Cytotoxic Steriods from the Mushroom Agaricus blazei.
Phytochamistry; 27(9):2777-2779 1988 12 Kawade M, Sumiya T, Shimura K, Ito H
Activation of the Reticuloendothelial System by Antitumor Polysaccharide from Agaricus
blazei Iwade. Igaku to Seibutsugaku; 109,299-302 1984 13 Mizuno T Medicinal
Functions of Reishi and Himematsutake. Sohgoh-Kyoiku-Kikaku, Tokyo; pp. 1-81
1990 14 Mizuno T Chemical Times; 131, 12 1989 15 Mizuno T, Ito H How Effective
Function of Himematsutake. Shozyu-sha, Tokyo; pp. 1-222 1987 16 Iwade T, Ito H
Miracle Himematsutake. Chikyu-sha, Tokyo; pp. 1-152. 1982 17 Ito H, Shimura K,
Japan Kokai Tokkyo Koho; 55, 74, 797 June 5, 1980 18 Ito H, Shimura K, Japan
Kokai Tokkyo Koho; 55, 108, 292-293 Aug 20, 1980 19 Mizuno T, Ito H, Shimura K,
Kawade M, Kawagishi H, Hagiwara T, Nakamura T Japan Kokai Tokkyo Koho; 64, 66,
127; 64, 67, 194; 64, 67, 195 March 13, 1989 20 Mizuno T, Ito H, Shimura K,
Kawade M, Kawagishi H, Hagiwara T, Nakamura T Japan Kokai Tokkyo Koho; 2, 78,
630 March 19, 1990 21 Mizuno T, Kawagishi H, Ito H, Shimura K Bull. Fac. Agr.
Shizuoka Univ.; 38, 29 1988 22 Mizuno T, Kawagishi H, Mizuno K Bull. Fac. Agr.
Shizuoka Univ.; 36, 85, 1989